Journal
SENSORS
Volume 12, Issue 1, Pages 347-358Publisher
MDPI
DOI: 10.3390/s120100347
Keywords
microwell; single-cell; cell lysis; microfluidics
Funding
- National Science Council of the Republic of China [NSC 99-2923-E-194-001-MY3]
- National Nano Device Laboratories of the Republic of China [NDL99-C02M3-064]
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Many conventional biochemical assays are performed using populations of cells to determine their quantitative biomolecular profiles. However, population averages do not reflect actual physiological processes in individual cells, which occur either on short time scales or nonsynchronously. Therefore, accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. Microfluidic chips with arrays of microwells were developed for single-cell chemical lysis in the present study. The cellular occupancy in 30-mu m-diameter microwells (91.45%) was higher than that in 20-mu m-diameter microwells (83.19%) at an injection flow rate of 2.8 mu L/min. However, most of the occupied 20-mu m-diameter microwells contained individual cells. The results of chemical lysis experiments at the single-cell level indicate that cell membranes were gradually lysed as the lysis buffer was injected; they were fully lysed after 12 s. Single-cell chemical lysis was demonstrated in the proposed microfluidic chip, which is suitable for high-throughput cell lysis.
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