Journal
SENSORS
Volume 10, Issue 3, Pages 2045-2053Publisher
MDPI
DOI: 10.3390/s100302045
Keywords
immunosensor; localized surface plasmon resonance (LSPR); gold nano-island; enzyme-catalyzed precipitation
Funding
- Center for Nanoscale Mechatronics and Manufacturing [08K1401-00313]
- Ministry of Education, Science and Technology, KOREA
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An enzymatic reaction was employed as a means to enhance the sensitivity of an immunosensor based on localized surface plasmon resonance (LSPR). The reaction occurs after intermolecular binding between an antigen and an antibody on gold nano-island (NI) surfaces. For LSPR sensing, the gold NI surface was fabricated on glass substrates using vacuum evaporation and heat treatment. The interferon-gamma (IFN-gamma) capture antibody was immobilized on the gold NIs, followed by binding of IFN-gamma to the antibody. Subsequently, a biotinylated antibody and a horseradish peroxidase (HRP) conjugated with avidin were simultaneously introduced. A solution of 4-chloro-1-naphthol (4-CN) was then used for precipitation; precipitation was the result of the enzymatic reaction catalyzed the HRP on gold NIs. The LSPR spectra were obtained after each binding process. Using this method, the enzyme-catalyzed precipitation reaction on the gold NI surface was found to effectively amplify the change in the signal of the LSPR immunosensor after intermolecular binding.
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