Journal
SENSORS
Volume 10, Issue 2, Pages 1377-1398Publisher
MDPI
DOI: 10.3390/s100201377
Keywords
biosensor; Pseudomonas F113; PCB; biodegradation
Funding
- EU [QLRT-2001-00101]
- DAF Stimulus Programme P-Solve
- Technological Sector Research programme under the HEA
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Whole-cell microbial biosensors are one of the newest molecular tools used in environmental monitoring. Such biosensors are constructed through fusing a reporter gene such as lux, gfp or lacZ, to a responsive promoter. There have been many reports of the applications of biosensors, particularly their use in assaying pollutant toxicity and bioavailability. This paper reviews the basic concepts behind the construction of whole-cell microbial biosensors for pollutant monitoring, and describes the applications of two such biosensors for detecting the bioavailability and biodegradation of Polychlorinated Biphenyls (PCBs).
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