Journal
SENSORS
Volume 10, Issue 2, Pages 1355-1376Publisher
MDPI
DOI: 10.3390/s100201355
Keywords
design strategy of fluorescent biosensors; biological macromolecular receptor; genetically encoded fluorescent biosensors; chemically modified protein based sensors; signaling aptamers
Funding
- Ministry of Education, Culture, Sports, Science, and Technology (Japan) [20241051]
- Grants-in-Aid for Scientific Research [20241051, 22750152] Funding Source: KAKEN
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Fluorescent biosensors to detect the bona fide events of biologically important molecules in living cells are increasingly demanded in the field of molecular cell biology. Recent advances in the development of fluorescent biosensors have made an outstanding contribution to elucidating not only the roles of individual biomolecules, but also the dynamic intracellular relationships between these molecules. However, rational design strategies of fluorescent biosensors are not as mature as they look. An insatiable request for the establishment of a more universal and versatile strategy continues to provide an attractive alternative, so-called modular strategy, which permits facile preparation of biosensors with tailored characteristics by a simple combination of a receptor and a signal transducer. This review describes an overview of the progress in design strategies of fluorescent biosensors, such as auto-fluorescent protein-based biosensors, protein-based biosensors covalently modified with synthetic fluorophores, and signaling aptamers, and highlights the insight into how a given receptor is converted to a fluorescent biosensor. Furthermore, we will demonstrate a significance of the modular strategy for the sensor design.
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