Journal
SENSOR LETTERS
Volume 10, Issue 3-4, Pages 886-893Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/sl.2012.2593
Keywords
Core-Shell Fe3O4@Au Nanoparticles; Alpha-Fetoprotein; Immunoassay; Electrochemical ELISA
Funding
- National Natural Science Foundation of China [20805024, 81072336]
- Natural Science Foundation of Ningbo [2011A610018, 2011A610006]
- K.C. Wong Magna Fund in Ningbo University, Science and Technology Planning Project of Guangdong Province [2010A030300006, 2008A050200006]
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A novel electrochemical enzyme-linked immunosorbent assay (ELISA) system of o-phenylenediamine (OPD)-H2O2-horseradish peroxidase (HRP) for sensitive detection of alpha-fetoprotein (AFP) was designed using magnetic core-shell Fe3O4@Au nanoparticles as labels. The detection antibodies were prepared by immobilization of HRP-anti-AFP and HRP onto the surface of Fe3O4@Au nanoparticles (Fe3O4@Au-HRP-anti-AFP). After the sandwich-type immunoreaction on polystyrene 96-well microtiter plate, the formed HRP-labeled immunoconjugate showed good enzymatic activity for the oxidation of OPD by H2O2. Enhanced detection sensitivity was achieved where the large surface area of Fe3O4@Au nanoparticles carriers increased the amount of HRP on per sandwich-type immunoreaction. The approach provided a linear response range between 0.01 and 200 ng . mL(-1) with a detection limit of 5 pg . mL(-1) The proposed electrochemical ELISA method exhibited good precision, high sensitivity and acceptable reproducibility, and could be further developed for clinical detections of AFP and other biomarkers.
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