Journal
SENSOR LETTERS
Volume 8, Issue 6, Pages 864-870Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/sl.2010.1357
Keywords
Hemoglobin; Amino-Silane Magnetic Nanoparticle; Hydrogen Peroxide; Biosensors
Funding
- National Natural Science Foundation of China [20975031]
- Foundation of the Education Department of Hubei Province, P.R. China [D20102501]
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Amino-silane magnetic nanoparticles (A-SMNPs) were mixed with graphite powder to construct A-SMNPs-contained carbon paste electrodes (CPE). Hemoglobin (Hb) was then immobilized on the electrode surface to form Hb/A-SMNPs/CPE biosensors. The biosensor was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The results indicated that Hb was stably immobilized on the electrode and exhibit excellent electrocatalytic activity to the reduction of hydrogen peroxide without the aid of an electron mediator. When used for amperometric measurement of H2O2, the prepared biosensor displayed good sensitivity, selectivity and stability. A linear detection range of H2O2 from 1.0 mu mol L-1 to 2.0 mmol L-1 and a detection limit of 0.27 mu mol L-1 (S/N = 3) was obtained. The apparent Michaelis-Menten constant was calculated to be 0.85 mmol L-1, indicating that the immobilized Hb had high enzymatic activity to H2O2.
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