A new electrochemical nitric oxide biosensor based on penicillamine self-assembled gold electrode

The direct electrochemical behavior of horse heart cytochrome c (Cyt. c) is studied by cyclic voltammetry (CV) at the gold electrode modified by penicillamine (PA). A pair of well-defined redox wave is investigated in the cytochrome c aqueous solution on the penicillamine/Au electrode. Cyt. c is immobilized on the penicillamine modified gold electrode by successive cyclic voltarnmetry to fabricate Cyt. c/PA/Au, which shows a well electrocatalytic response for the oxidation of NO. Cyt. c/PA/Au can be applied to the determination of trace NO.