Preparation and Characterization of Glucose Biosensors Using Self-Assembled Monolayers of Alkanethiols

Glucose biosensors were prepared by immobilizing glucose oxidase on gold electrodes modified with a self-assembled monolayer (SAM) of 3-mercaptopropionic acid (MPA) in this study. The important parameters in this fabrication process were investigated and the optimal fabrication conditions were selected. Using the glucose biosensors manufactured by the optimal procedure, the glucose concentration measurements were performed by chronoamperometry, where the optimal operating potential and time were selected. Also, the effect of mediator concentration on the sensitivity of the SAMs based glucose biosensors was investigated.