Journal
SCIENTIFIC REPORTS
Volume 5, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep14269
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Funding
- Global Frontier Project grant from the National Research Foundation (NRF) - Ministry of Science, ICT and Future Planning (MSIP) of Korea [NRF-2013M3A6A4045452]
- Basic Science Research Program through the NRF by the Ministry of Education [NRF-2015R1D1A3A01015641]
- Leaders Industry-University Cooperation'' Project by the Ministry of Education
- Basic Science Research Program from the NRF - Ministry of Education [2011-0021845]
- Korea Basic Science Institute grant [T35773]
- National Research Foundation of Korea [2011-0021845, 2015R1D1A3A01015641] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Targeted protein degradation is a powerful tool in determining the function of specific proteins or protein complexes. We fused nanobodies to SPOP, an adaptor protein of the Cullin-RING E3 ubiquitin ligase complex, resulting in rapid ubiquitination and subsequent proteasome-dependent degradation of specific nuclear proteins in mammalian cells and zebrafish embryos. This approach is easily modifiable, as substrate specificity is conferred by an antibody domain that can be adapted to target virtually any protein.
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