Journal
SEED SCIENCE RESEARCH
Volume 20, Issue 2, Pages 79-87Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0960258510000085
Keywords
Arabidopsis; cascades; microRNA; miR156; miR172
Categories
Funding
- NSF [IBN-0237562]
- American Seed Research Foundation
Ask authors/readers for more resources
MicroRNAs (miRNAs) are involved in developmental programmes of plants, including seed germination and post-germination. Here, we provide evidence that two different miRNA pathways, miR156 and miR172, interact during the post-germination stages in Arabidopsis. Mutant seedlings expressing miR156-resistant SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE13 (mSPL13), which has silent mutations in the miR156 complementary sequence, over-accumulated SPL13 mRNA and exhibited a delay in seedling development. Microarray analysis indicated that SCHNARCHZAPFEN (SNZ), an AP2-like gene targeted by miR172, was down-regulated in these mutants. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) and miRNA gel blot analyses showed that the MIR172 genes were up-regulated in mSPL13 mutants. These results suggest that the miRNA regulation cascades (miR156 H SPL13 -> miR172 SNZ) play a critical role during the post-germination developmental stages in Arabidopsis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available