Journal
SEED SCIENCE AND TECHNOLOGY
Volume 39, Issue 1, Pages 259-264Publisher
ISTA-INT SEED TESTING ASSOC
DOI: 10.15258/sst.2011.39.1.28
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- Krick House Trust, UK
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Hybrid purity is one of the most important characteristics of good quality seed. In order to identify pure hybrids and pollen shedders/offtypes an investigation was done to identify an ideal SSR marker. 58 primer pairs were screened to identify specific markers associated with each hybrid and parental lines. Hybrid KBSH-44 could be clearly identified by using 'ORS 309 and ORS 170' based on the banding pattern resolved on Polyacrylamide gel (6%). The complementary banding pattern of both parents identified the hybrid. ORS 309 amplified allele size of 250bp was specific to the female parent (CMS-17A) and 230 bp was specific to the male parent (RHA 95-C-1). These two bands of allele size 230 and 250bp were found in hybrid KBSH-44 only. Another SSR primer 'ORS 170' was able to distinguish the hybrid KBSH-44 by amplifying allele of size 230bp a female specific (CMS-17A) allele and 200 bp amplicon a male specific allele (RHA 95-C-1). SSR primer ORS 811 found specific to identify KBSH-53 and it amplified allele of size 270bp in its female parent (CMS-53A) and allele size of 230bp in its pollen parent (RHA 95-C-1). The hybrid has both the alleles from its parents at 270 and 230bp.
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