Journal
SCIENTIA HORTICULTURAE
Volume 164, Issue -, Pages 1-8Publisher
ELSEVIER
DOI: 10.1016/j.scienta.2013.08.018
Keywords
Capsaicin; Capsicum; Cotyledon; Naga King Chili; Putrescine; Scoville Heat Units
Categories
Funding
- University Grant Commission (UGC)
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An in vitro plantlet regeneration system has been developed from cotyledon segments of Capsicum chinense Jacq. cv. Naga King Chili, a very pungent chili species of India. Rosette-like structures (RLS) were induced on the explants which on transfer to medium containing indole-3-acetic acid (IAA) resulted in multiple shoots in course of time. Agar-based Murashige and Skoog (MS) medium fortified with 18.16 mu M thidiazuron (TDZ) was found to be the most suitable medium for RLS induction and shoot formation. Maximum number of multiple shoots (9.5 +/- 0.39) and roots (8.6 +/- 0.50), and root length (2.4 +/- 0.02 cm) were obtained from rosette-like structures in medium containing 5.70 mu M IAA. Combined effect of putrescine (Put) (5.6 mu M) and TDZ (4.54 mu M) mediated direct multiple shoot (5.8 +/- 0.44) induction. The in vitro rooted plantlets were transferred to glass house for hardening and acclimatization, wherein 90% survival was recorded. The hardened plantlets, thus developed were established in soil and bore normal fruits after 4 months of transfer. The capsaicin content in matured fruitS of in vitro propagated plants was found to be 0.05236 g/g d wt (837,760 Scoville Heat Units, SHU), where as in vivo raised plantlets yielded 0.0545 g/g d wt (872,000 SHU) respectively. (C) 2013 Elsevier B.V. All rights reserved.
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