Journal
SCIENTIA HORTICULTURAE
Volume 163, Issue -, Pages 20-26Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scienta.2013.07.018
Keywords
Citrus; Somatic cybridization; Symmetric fusion; Seedlessness; Genetic improvement
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Funding
- National Natural Science Foundation of China
- Ministry of Science & Technology of China [2011CB100606, 2011AA100205]
- Fundamental Research Funds for the Central Universities [2013PY045]
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Somatic hybridization holds great potential in citrus improvement by circumventing several biological barriers such as nucellar polyembryony, long juvenility and pollen/ovule sterility. To date more than 300 intergeneric and interspecific citrus somatic hybrids have been produced by standard symmetric fusion; among which, more than forty parental combinations produced leaf-parent-type cybrids or putative cybrids. Herein somatic cybrid production by protoplast fusion aiming to produce citrus cybrids with male sterile cytoplasm being transferred simply via symmetric fusion is detailed including methods to produce cybrids, possible mechanism and factors involved in cybrid production via symmetric fusion, strategy of targeted cybrid production for potential seedlessness, and agronomic performance and omics-based studies of some selected cybrids. This review provides the most comprehensive and updated information concerning citrus cybrid production over the past 20 years. (C) 2013 Elsevier B.V. All rights reserved.
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