Effect of 24-epibrassinolide on growth, yield, antioxidant system and cadmium content of bean (Phaseolus vulgaris L.) plants under salinity and cadmium stress

The plants of Phaseolus vulgaris L were grown in the presence of NaCl and/or CdCl2 and were sprayed with 5 mu M of 24-epibrassinolide (EBL) at 15 days after transplanting (DAT) and were sampled at 30 DAT and at the end of experiment. The plants exposed to NaCl and/or CdCl2 exhibited a significant decline in growth, the level of pigment parameters, green pod yield and pod protein. However, the follow up treatment with EBL detoxified the stress generated by NaCl and/or CdCl2 and significantly improved the above parameters. The NaCl and/or CdCl2 increased electrolyte leakage, lipid peroxidation and plant Cd2+ content, and decreased the membrane stability index (MSI) and relative water content. However, the EBL treatment in absence of the stress improved the MSI and relative water content and minimized plant Cd2+ content but could not influence electrolyte leakage and lipid peroxidation. The antioxidative enzymes and the level of proline exhibited a significant increase in response to EBL as well as to NaCl and/or CdCl2 stress. (C) 2011 Elsevier B.V. All rights reserved.