Journal
SCIENTIA HORTICULTURAE
Volume 119, Issue 3, Pages 330-334Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scienta.2008.07.036
Keywords
Cauliflower; Shoot regeneration; Protoplast culture
Categories
Funding
- European Union 5th Framework [QLK3CT1999013692]
- RMIT University
Ask authors/readers for more resources
Adventitious shoot regeneration and protoplast isolation and culture were examined from leaf explants of in vitro shoot cultures of several cauliflower (Brassica oleracea var. botrytis) cultivars, sourced from Europe and Australia, was investigated with the aim to develop improved nuclear and plastid transformation protocols for this vegetable crop. Eight out of 10 cultivars regenerated shoots from at least 79% of leaf explants. Mesophyll protoplasts from leaves gave high yields and division frequencies. Growth of shoot cultures in large glass vessels with vented lids was the key factor in obtaining high protoplast division frequencies of up to 71% and at least 70% of protoplast calluses regenerating shoots. Crown Copyright (c) 2008 Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available