Amelioration of chilling stress by paclobutrazol in watermelon seedlings

Paclobutrazol (PBZ) is a member of the triazole plant-growth inhibitor group that is responsible for inducing tolerance to number of biotic and abiotic stresses. An experiment was, therefore, conducted to test whether PBZ application at various concentrations (0, 25, 50 and 75 mg L-1) through seed soaking or foliar spray Would protect watermelon (Citrullus lanatus) seedlings, subjected to chilling stress. Thirty-five-day old plants were exposed to chilling 5 h/day at 4 degrees C for 5 days. PBZ improved growth rate of watermelon seedling subjected to chilling stress and increased relative leaf chlorophyll content (RLCC) and chlorophyll fluorescence ratio (Fv/Fm) compared with the control at the end of chilling stress. PBZ ameliorated the injury caused by chilling stress by inhibiting increases in proline and leaf electrolyte leakage. which suggested that PBZ ameliorated the negative effect of chilling stress. PBZ was most effective in increased chilling tolerance of watermelon seedling when applied using the seed soak method than as a foliar spray. The best protection appeared to be obtained from seedlings seed soaked with PBZ at 50 and 75 mg L-1. (c) 2009 Elsevier B.V. All rights reserved,