Journal
SCIENTIA HORTICULTURAE
Volume 117, Issue 1, Pages 1-8Publisher
ELSEVIER
DOI: 10.1016/j.scienta.2008.01.012
Keywords
mitogen-activated protein kinase; cucumber; excess NO3-; abiotic stresses
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Mitogen-activated protein kinases (MAPKs) play important roles in the transduction of extracellular signals to the intracellular targets in all eukaryotes. Here, a cucumber cDNA designated CsNMAPK, encoding a mitogen-activated protein kinase was isolated using RT-PCR, 3' and 5' RACE. The full-length cDNA sequence contains 1636 bp and an open reading frame (ORF) of 1113 bp, which encodes 370 amino acid residues. According to the phylogenetic analysis, CsNMAPK belongs to subgroup I MAPK in plants. Northern blot analysis revealed that CsNMAPK expressed differently in response to excess NO3-. And the CsNMAPK expression kinetics between a salt-resistant cultivar (Xintaimici) and a salt-sensitive cultivar (Shennongchunwu) was slightly different under 182 mmol L-1 NO3 treatment. The mRNA levels also increased after 24 h treatments with H2O2 and salicylic acid (SA), but decreased with abscisic acid (ABA) and low-temperature. However, there was no significant induction of CsNMAPK gene after 24 h drought and high-temperature treatments. Our results suggested that a MAP kinase cascade may function in excess NO3- and other abiotic stresses in cucumber. (c) 2008 Published by Elsevier B.V.
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