Journal
SCIENCEASIA
Volume 38, Issue 3, Pages 319-322Publisher
THAILANDS NATL SCIENCE & TECHNOLOGY DEVELOPMENT AGENCY
DOI: 10.2306/scienceasia1513-1874.2012.38.319
Keywords
minimal inhibitory concentration (MIC); Gram positive bacteria; Gram negative bacteria; total phenolic content
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The in vitro antibacterial activities of different extracts of pomegranate fruit peels and arils (with seeds) were investigated by agar-well diffusion and broth dilution methods against four food-related bacteria (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium). The solvents used as extractants in this study were hot water, 95% ethanol, and acetone. Their total phenolic contents were also evaluated. All pomegranate extracts contained high levels of phenolics and exhibited antibacterial activity against all bacteria tested. The hot-water extract of the peels was the most potent with the minimal inhibitory concentration of 207 mg/ml against E. coli and less than 103.6 mg/ml against the other bacteria. Gram-positive bacteria were generally more sensitive to the extracts than Gram-negative ones.
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