Journal
SCIENCE TRANSLATIONAL MEDICINE
Volume 3, Issue 83, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/scitranslmed.3002076
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Funding
- Department of Health via the National Institute for Health Research (NIHR) comprehensive Biomedical Research Centre
- King's College London
- King's College Hospital NHS Foundation Trust
- MRC Centre for Transplantation
- British Heart Foundation, NIH [RO1AR040065]
- Wellcome Trust [GR078173MA]
- Guy's & St Thomas's Charity
- British Heart Foundation [RG/08/005/25303] Funding Source: researchfish
- Medical Research Council [G0601387] Funding Source: researchfish
- MRC [G0601387] Funding Source: UKRI
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Graft rejection by the immune system is a major cause of transplant failure. Lifelong immunosuppression decreases the incidence of graft rejection; however, nonspecific immunosuppression results in increased susceptibly to infection and cancer. Regulatory T cells (T-regs), which suppress the activation of the immune system and induce tolerance, are currently under evaluation for use in clinical transplantation. Ex vivo expanded polyclonal T-regs that are introduced into transplant recipients alter the balance of T effector cells to T-regs; however, experimental data suggest that alloantigen-specific T-regs would be more effective at preventing graft rejection. We have developed a method to enrich alloantigen-specific human T-regs based on the coexpression of activation markers, CD69 and CD71. These T-regs could be readily expanded in vitro and demonstrated potent antigen-specific suppression. In a humanized mouse model of alloimmune-mediated injury of human skin grafts, alloantigen-specific T-regs resulted in a significant reduction in clinically relevant indicators of dermal tissue injury when compared with polyclonal T-regs, restoring a histology comparable to healthy skin. This method of human allospecific T-reg selection should be scalable to the clinic. The improved in vivo efficacy of alloantigen-specific T-regs over polyclonal T-regs shown here suggests that generating customized T-regs with defined anti-donor allospecificities may improve current practice in clinical immunotherapy.
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