Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 470, Issue -, Pages 983-992Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scitotenv.2013.10.076
Keywords
Biodegradation pathway; High-throughput sequencing; Sediment; DDT; HCH; Pesticide
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Funding
- Research Grants Council of the Hong Kong Special Administrative Region, China [HKU7190/12E]
- Hong Kong Environment Conservation Fund [ECF 35/2009]
- National Natural Science Foundation of China [21377112]
- National High Technology R&D Program of China [2012AA06A204]
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The abundance and diversity of biodegradation genes (BDGs) and potential degradation pathways of dichlorodiphenyltrichloroethane (DDT), hexachlorocydohexane (HCH), and atrazine (ATZ) in freshwater and marine sediments were investigated by metagenomic analysis using 6 datasets (16 Gb in total). The datasets were derived using Illumina high-throughput sequencing and were based on BLAST against self-established databases of BDGs, DDT degradation genes (DDGs), HCH degradation genes (HDGs), and ATZ degradation genes (ADGs). The results showed that the abundance and diversity of BDGs, DDGs, HDGs, and ADGs varied with sample source and locations. The lip and mnp genes, which encode for peroxidase, and the carA gene, which encodes for laccase, were detected as the dominant genes for degradation of organic pollutants. The hdt, hdg, and atzB genes, which encode for hydratase, dehalogenase, and ethylaminohydrolase, were found to be the most abundant genes involved in DDT, HCH, and ATZ degradation, respectively. The identified 69 genera capable of degrading organic pollutants were mostly affiliated with Proteobacteria (49.3%) and Actinobacteria (21.7%). Four genera, including Plesiocystis, Anaerolinea, Jannaschia, and Mycobacterium, were the major biodegradation populations in all sediments. In this study, the nearly complete biodegradation pathways of DDT and ATZ were found, and the partial degradation pathway of HCH was detected in all sediments. (C) 2013 Elsevier B.V. All rights reserved.
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