Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 482, Issue -, Pages 116-124Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2014.02.125
Keywords
Toxic Alexandrium tamarense; Algicidal bacteria; Algicidal substance; Algicidal mechanism; HABs control
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Funding
- National Nature Science Foundation of China [40930847, 41376119]
- Public Science and Technology Research Funds Projects of Ocean [201305016, 201305022]
- Program for Changjiang Scholars and Innovative Research Team in University [41121091]
- Xiamen University MEL Young Scientist Visiting Fellowship [MELRS1124]
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Algicidal bacteria may play a major role in controlling harmful algal blooms (HABs) dynamics. Bacterium DH77-1 was isolated with high algicidal activity against the toxic dinoflagellate Alexandrium tamarense and identified as Joostella sp. DH77-1. The results showed that DH77-1 exhibited algicidal activity through indirect attack, which excreted active substance into the filtrate. It had a relatively wide host range and the active substance of DH77-1 was relatively stable since temperature, pH and storage condition had no obvious effect on the algicidal activity. The algicidal compound from bacterium DH77-1 was isolated based on activity-guided bioassay and the molecular weight was determined to be 125.88 by MALDI-TOF mass spectrometer, however further identification via nuclear magnetic resonance (NMR) spectra is ongoing. The physiological responses of algal cells after exposure to the DH77-1 algicidal substances were as follows: the antioxidant system of A. tamarense responded positively in self-defense; total protein content decreased significantly as did the photosynthetic pigment content; superoxide dismutase, peroxidase enzyme and malondialdehyde content increased extraordinarily and algal cell nucleic acid leaked seriously ultimately inducing cell death. Furthermore, DH77-1 is the first record of a Joostella sp. bacterium being algicidal to the harmful dinoflagellate A. tamarense, and the bacterial culture and the active compounds might be potentially used as a bio-agent for controlling harmful algal blooms. (C) 2014 Elsevier B.V. All rights reserved.
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