Journal
SCIENCE CHINA-LIFE SCIENCES
Volume 53, Issue 2, Pages 292-300Publisher
SCIENCE PRESS
DOI: 10.1007/s11427-010-0048-x
Keywords
mulberry enterobacter wilting; Enterobacter; phenotype identification; ERIC-PCR; 16S rRNA
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Funding
- National Natural Science Foundation of China [30871655, 30671397]
- National High Technology Research and Development Project [2006AA10A211]
- Zhejiang Provincial Agricultural Department [ZNJF2008-97]
- Hangzhou Science & Technology Bureau and Hangzhou Agricultural Development Foundation [2008-3]
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Thirty-six pathogenetic bacterial strains were isolated from wilted mulberry plants in Hangzhou, Zhejiang province of China. The six representative strains were confirmed to be involved in more than one Enterobacter species by common bacteriological test, electron microscope observation, hypersensitive reaction, Koch's postulates, physiological and biochemical test, biolog, fatty acid methyl esters analysis (FAMEs), enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR), 16s rRNA sequences analysis, and comparative analysis with 7 type strains and 3 reference strains. This is the first report on mulberry disease caused by Enterobacter spp. in the world providing new evidence on induction of the plant disease in this genus. The results are not only important in the mulberry disease management but also have significant scientific value for further studies of opportunistic human pathogens and environmental strains in Enterobacter.
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