Journal
SCIENCE CHINA-CHEMISTRY
Volume 54, Issue 3, Pages 515-520Publisher
SCIENCE PRESS
DOI: 10.1007/s11426-010-4200-z
Keywords
molecular imprinting; microgels; esterase; temperature sensitivity; dextran
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Funding
- National Natural Science Foundation of China [21074152, 20874116, 20676155, J0730420]
- Natural Science Foundation of Guangdong Province in China [8151027501000004, 9151027501000105]
- Ministry of Education Ministry of China [20090171110023]
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Temperature-sensitive molecularly imprinted microgels (MIGs) exhibiting esterase activity were prepared by a reverse emulsion method using dialdehyde dextran-histidine conjugate (PAD-His) as the functional macromonomer and p-nitrophenyl phosphate (NPP) as the stable transition state analogue (TSA) as well as Co2+ as the coordination center. The catalytic activity of MIGs was greatly influenced by the amount of the template, and could be modulated by temperature. The hydrolysis kinetics of p-nitrophenyl acetate (NPA) in the presence of MIGs could be described by the Michaelis-Menten equation. The Michaelis-Menten constant and maximum velocity were found to be 2.2 x 10(-5) mol/L and 2.04 x 10(a8) mol/h, respectively. In addition, the MIGs were found to have a high catalytic selectivity to NPA.
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