Journal
SCIENCE CHINA-CHEMISTRY
Volume 54, Issue 8, Pages 1298-1303Publisher
SCIENCE PRESS
DOI: 10.1007/s11426-011-4326-7
Keywords
abscisic acid; immunomagnetic assay; chemiluminescence; CdSe/ZnS
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Funding
- National Natural Science Foundation of China [21075093, 90717111, 20921062]
- National Key Scientific Program-Nanoscience and Nanotechnology [2011CB933600]
- National Basic Research Program of China [2007CB714507]
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Abscisic acid (ABA) is an important plant hormone. It plays a key role in regulating plant responses to abiotic stress and in controlling seed germination, growth, and stomatal aperture. A rapid, sensitive analytical method for the ABA detection is urgently required for further investigation of ABA signaling. In this work, an immunomagnetic assay combined with CdSe/ZnS amplification of chemiluminescence has been developed for the detection of ABA. The result could be read out in 30 min at least, with the simplified procedure of immunomagnetic assay. Under the optimized condition, a linear range from 1 pM to 10 nM was obtained. An unexpected result induced by the dose hook effect was discussed. This method provided the high selectivity for ABA over other components that might be contained in real samples.
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