Journal
SCIENCE CHINA-CHEMISTRY
Volume 53, Issue 6, Pages 1366-1370Publisher
SCIENCE PRESS
DOI: 10.1007/s11426-010-3091-3
Keywords
Vibrio parahaemolyticus; electrochemical DNA sensor; self-assembling; gold electrode
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Funding
- Shanghai Nano-Technology Promotion Center [0752nm025]
- Ministry of Science and Technology of the People's Republic of China [0424]
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An electrochemical DNA biosensor for specific-sequences detection of Vibrio parahaemolyticus (VP) was fabricated. A single-stranded 20-mer oligonucleotide (ssDNA) and 6-mercapto-1-hexanol (MCH) were immobilized via a thiol linker on gold disk electrodes by self-assembling. The ssDNA underwent hybridization in a hybridization solution containing complementary or non-complementary or single base pair mismatched DNA sequences of VP. Examination of changes in response to these three target DNAs showed that the developed biosensor had a high selectivity and sensitivity.
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