4.6 Article

Terminal alkyne substituted O-6-benzylguanine for versatile and effective syntheses of fluorescent labels to genetically encoded SNAP-tags

Journal

RSC ADVANCES
Volume 5, Issue 30, Pages 23646-23649

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra17072e

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Funding

  1. National Natural Science Foundation of China [21174022, 21376038, 21421005]
  2. National Basic Research Program of China [2013CB733702]
  3. Program for Changjiang Scholars and Innovative Research Team in University [IRT1213]
  4. Fundamental Research Funds for the Central Universities [DUT14YQ103]
  5. Specialized Research Fund for the Doctoral Program of Higher Education [20110041110009]

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We have developed a terminal alkyne substituted O6-benzylguanine, named PYBG, as a versatile precursor to be facilely conjugated with various fluorescent dyes through 'Click chemistry' and Sonogashira coupling reactions. These fluorescent PYBG derivatives specifically and efficiently label the target genetically encoded SNAP-tags in live cells.

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