Journal
SCIENCE
Volume 361, Issue 6409, Pages 1386-+Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aat8849
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Funding
- NIH [R35GM118015]
- NSFC [31521002, MOST 2017YFA0103304, CAS XDB08010100]
- Cancerfonden and the Swedish Research Council
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How parental histone (H3-H4)(2) tetramers, the primary carriers of epigenetic modifications, are transferred onto leading and lagging strands of DNA replication forks for epigenetic inheritance remains elusive. Here we show that parental (H3-H4)(2) tetramers are assembled into nucleosomes onto both leading and lagging strands, with a slight preference for lagging strands. The lagging-strand preference increases markedly in budding yeast cells lacking Dpb3 and Dpb4, two subunits of the leading strand DNA polymerase, Pol epsilon, owing to the impairment of parental (H3-H4)(2) transfer to leading strands. Dpb3-Dpb4 binds H3-H4 in vitro and participates in the inheritance of heterochromatin. These results indicate that different proteins facilitate the transfer of parental (H3-H4)(2) onto leading versus lagging strands and that Dbp3-Dpb4 plays an important role in this poorly understood process.
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