Journal
SCIENCE
Volume 344, Issue 6185, Pages 711-716Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1251358
Keywords
-
Categories
Funding
- National Science Foundation (NSF) Arabidopsis grant [MCB-0618402, MCB-1052348]
- [NSF-MCB-1021677]
- [NSF-MCB-0918220]
- [NSF-MCB-1121612]
- Direct For Computer & Info Scie & Enginr
- Div Of Information & Intelligent Systems [1161007] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1052348, 1244303, 1414339, 0918220] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1121612] Funding Source: National Science Foundation
Ask authors/readers for more resources
Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 x 10(6) pairs, we identified 12,102 membrane/signaling protein interactions from Arabidopsis. Besides confirmation of expected interactions such as heterotrimeric G protein subunit interactions and aquaporin oligomerization, >99% of the interactions were previously unknown. Interactions were confirmed at a rate of 32% in orthogonal in planta split-green flourescent protein interaction assays, which was statistically indistinguishable from the confirmation rate for known interactions collected from literature (38%). Regulatory associations in membrane protein trafficking, turnover, and phosphorylation include regulation of potassium channel activity through abscisic acid signaling, transporter activity by a WNK kinase, and a brassinolide receptor kinase by trafficking-related proteins. These examples underscore the utility of the membrane/signaling protein interaction network for gene discovery and hypothesis generation in plants and other organisms.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available