Journal
SCIENCE
Volume 346, Issue 6208, Pages 477-481Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1253585
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Funding
- U.K. Medical Research Council Career Development Award [MR/K007106/1]
- Cancer Research UK Birmingham Centre award [DF/270312]
- ERASMUS scholarship
- Wellcome Trust strategic award [ISSFPP47]
- Cancer Research UK Birmingham Centre [CAICS/05/12]
- Medical Research Council [MR/K007106/1] Funding Source: researchfish
- MRC [MR/K007106/1] Funding Source: UKRI
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Resolution of replication forks during termination of DNA replication is essential for accurate duplication of eukaryotic genomes. Here we present evidence consistent with the idea that polyubiquitylation of a replisome component (Mcm7) leads to its disassembly at the converging terminating forks because of the action of the p97/VCP/Cdc48 protein remodeler. Using Xenopus laevis egg extract, we have shown that blocking polyubiquitylation results in the prolonged association of the active helicase with replicating chromatin. The Mcm7 subunit is the only component of the active helicase that we find polyubiquitylated during replication termination. The observed polyubiquitylation is followed by disassembly of the active helicase dependent on p97/VCP/Cdc48. Altogether, our data provide insight into the mechanism of replisome disassembly during eukaryotic DNA replication termination.
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