Journal
SCIENCE
Volume 343, Issue 6174, Pages 1010-1014Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1249484
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Funding
- Fibrolamellar Cancer Foundation
- Rockefeller University Center for Clinical and Translational Science [2UL1RR024143]
- Howard Hughes Medical Institute International Student Predoctoral Fellowship
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Fibrolamellar hepatocellular carcinoma (FL-HCC) is a rare liver tumor affecting adolescents and young adults with no history of primary liver disease or cirrhosis. We identified a chimeric transcript that is expressed in FL-HCC but not in adjacent normal liver and that arises as the result of a similar to 400-kilobase deletion on chromosome 19. The chimeric RNA is predicted to code for a protein containing the amino-terminal domain of DNAJB1, a homolog of the molecular chaperone DNAJ, fused in frame with PRKACA, the catalytic domain of protein kinase A. Immunoprecipitation and Western blot analyses confirmed that the chimeric protein is expressed in tumor tissue, and a cell culture assay indicated that it retains kinase activity. Evidence supporting the presence of the DNAJB1-PRKACA chimeric transcript in 100% of the FL-HCCs examined (15/15) suggests that this genetic alteration contributes to tumor pathogenesis.
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