Journal
RSC ADVANCES
Volume 5, Issue 39, Pages 30687-30693Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ra01566a
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- Thailand's Office of the Higher Education Commission through the National Research University (NRU) Project for Chiang Mai University (CMU)
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Herein, magnetic-EpCAM nanoparticle (EpCAM-MNP) was developed and exploited as nanoprobe for targeting, isolating and imaging hepatocellular carcinoma. The nanoprobe was composed of two major components including aminosilane-coated iron oxide nanoparticles and DNA-based EpCAM aptamers. Extensive studies were carried out to investigate its physico-chemical properties, magnetic relaxivity, as well as biocompatibility. The results indicated that the small size of the nanoprobe (HD < 100 nm) had high R-2 relaxivity with good biocompatibility. Study on cellular accumulation and cellular uptake demonstrated high accumulation of EpCAM-MNP that was internalized via receptor-mediated endocytosis, as evidenced by TEM analysis. By using a magnetic-activated cell sorting (MACS) approach, the majority (similar to 96%) of the HepG2 cells were isolated, indicating the feasibility of our nanoprobe for isolating EpCAM-positive cells in clinical application. Because of the high intracellular accumulation and the high R-2 relaxivity of EpCAM-MNP, it can be used for quantitative MRI detection of cancer cells with high sensitivity.
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