Journal
SCIENCE
Volume 346, Issue 6208, Pages 440-+Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1253596
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Funding
- Cancer Research UK
- Medical Research Council
- Wellcome Trust [097945/B/11/Z, 102943/Z/13/Z]
- MRC [MC_UU_12016/13] Funding Source: UKRI
- Wellcome Trust [102943/Z/13/Z] Funding Source: Wellcome Trust
- Cancer Research UK [16326] Funding Source: researchfish
- Medical Research Council [MC_UU_12016/13] Funding Source: researchfish
- Wellcome Trust [102943/Z/13/Z] Funding Source: researchfish
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Chromosome replication is initiated by a universal mechanism in eukaryotic cells, involving the assembly and activation at replication origins of the CMG (Cdc45-MCM-GINS) DNA helicase, which is essential for the progression of replication forks. Disassembly of CMG is likely to be a key regulated step at the end of chromosome replication, but the mechanism was unknown until now. Here we show that the ubiquitin ligase known as SCFDia2 promotes ubiquitylation of CMG during the final stages of chromosome replication in Saccharomyces cerevisiae. The Cdc48/p97 segregase then associates with ubiquitylated CMG, leading rapidly to helicase disassembly. These findings indicate that the end of chromosome replication in eukaryotes is controlled in a similarly complex fashion to the much-better-characterized initiation step.
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