Journal
SCIENCE
Volume 346, Issue 6213, Pages 1077-+Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1258096
Keywords
-
Categories
Funding
- Alexander von Humboldt Foundation
- German Federal Ministry for Education and Research
- Helmholtz Association
- German Research Foundation
- Goran Gustafsson Foundation
- Swedish Research Council
- Kempe Foundation
- Umea University
- Howard Hughes Medical Institute
- NSF
- Gates Foundation
- Li Ka Shing Foundation
- NIH
Ask authors/readers for more resources
The advent of facile genome engineering using the bacterial RNA-guided CRISPR-Cas9 system in animals and plants is transforming biology. We review the history of CRISPR (clustered regularly interspaced palindromic repeat) biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism, which has set the stage for remarkable developments using this technology to modify, regulate, or mark genomic loci in a wide variety of cells and organisms from all three domains of life. These results highlight a new era in which genomic manipulation is no longer a bottleneck to experiments, paving the way toward fundamental discoveries in biology, with applications in all branches of biotechnology, as well as strategies for human therapeutics.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available