Journal
RSC ADVANCES
Volume 5, Issue 29, Pages 22361-22364Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra16917d
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Funding
- Major State Basic Research Development Program of China (973 Program) [2012CB822102]
- National High Technology Research and Development Program of China (863 Program) [2012AA021504]
- National Major Scientific and Technological Special Project for Significant New Drugs Development [2012ZX09502001-005]
- Independent Innovation Foundation of Shandong University [2012TS014]
- Keygrant Project of Chinese Ministry of Education [313033]
- Science and Technology Development Project of Shandong Province [2014GSF121006]
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Directed evolution of an alpha-galactosidase (Aga2) from Bifidobacterium breve 203 by random mutagenesis and subsequently by site-directed mutagenesis provided a mutant enzyme V564N that showed high alpha-transgalactosylation efficiency with unobserved hydrolysis towards transglycosylation products. Using this enzyme, a one-step reaction for the simultaneous synthesis of alpha-Gal epitope and globotriose derivatives was achieved.
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