Journal
SCIENCE
Volume 339, Issue 6123, Pages 1074-1077Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1232542
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Funding
- European Research Council (ERC) Starting Grant [242922]
- Boehringer Ingelheim GmbH
- European Research Council (ERC) [242922] Funding Source: European Research Council (ERC)
- Austrian Science Fund (FWF) [W1207] Funding Source: Austrian Science Fund (FWF)
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Genomic enhancers are important regulators of gene expression, but their identification is a challenge, and methods depend on indirect measures of activity. We developed a method termed STARR-seq to directly and quantitatively assess enhancer activity for millions of candidates from arbitrary sources of DNA, which enables screens across entire genomes. When applied to the Drosophila genome, STARR-seq identifies thousands of cell type-specific enhancers across a broad continuum of strengths, links differential gene expression to differences in enhancer activity, and creates a genome-wide quantitative enhancer map. This map reveals the highly complex regulation of transcription, with several independent enhancers for both developmental regulators and ubiquitously expressed genes. STARR-seq can be used to identify and quantify enhancer activity in other eukaryotes, including humans.
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