Journal
SCIENCE
Volume 339, Issue 6119, Pages 576-579Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1231887
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Funding
- National Institute on Allergy and Infection, NIH [R01AI087946]
- Welch Foundation [AU-1714]
- NIH [R01GM61074]
- Human Frontier Science Program
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Adsorption and genome ejection are fundamental to the bacteriophage life cycle, yet their molecular mechanisms are not well understood. We used cryo-electron tomography to capture T7 virions at successive stages of infection of Escherichia coli minicells at similar to 4-nm resolution. The six phage tail fibers were folded against the capsid, extending and orienting symmetrically only after productive adsorption to the host cell surface. Receptor binding by the tail triggered conformational changes resulting in the insertion of an extended tail, which functions as the DNA ejection conduit into the cell cytoplasm. After ejection, the extended phage tail collapsed or disassembled, which allowed resealing of the infected cell membrane. These structural studies provide a detailed series of intermediates during phage infection.
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