Journal
SCIENCE
Volume 338, Issue 6111, Pages 1229-1232Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1228745
Keywords
-
Categories
Funding
- NIH [1RC4GM096355-01, 5R01GM061126-12, 5T32-HD055200, 5R01GM067858]
- Intellectual and Developmental Disabilities Research Center [NIH 5P30HD024064]
- Nakajima Foundation
- Taiwan Merit Scholarships Program
- National Science Council [NSC-095-SAF-I-564-015-TMS]
- Edward and Josephine Hudson Scholarship Fund
- Houston Laboratory and Population Science Training Program in Gene-Environment Interaction from the Burroughs Wellcome Fund [1008200]
- Research Education and Career Horizon Institutional Research and Academic Career Development Award Fellowship [5K12GM084897]
Ask authors/readers for more resources
Notch signaling affects many developmental and cellular processes and has been implicated in congenital disorders, stroke, and numerous cancers. The Notch receptor binds its ligands Delta and Serrate and is able to discriminate between them in different contexts. However, the specific domains in Notch responsible for this selectivity are poorly defined. Through genetic screens in Drosophila, we isolated a mutation, Notch(jigsaw), that affects Serrate-but not Delta-dependent signaling. Notch(jigsaw) carries a missense mutation in epidermal growth factor repeat-8 (EGFr-8) and is defective in Serrate binding. A homologous point mutation in mammalian Notch2 also exhibits defects in signaling of a mammalian Serrate homolog, Jagged1. Hence, an evolutionarily conserved valine in EGFr-8 is essential for ligand selectivity and provides a molecular handle to study numerous Notch-dependent signaling events.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available