Journal
SCIENCE
Volume 336, Issue 6085, Pages 1171-1174Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1219364
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Funding
- DOE Biological and Environmental Research (DOE-BER) [DE-FC03-02ER63421]
- National Center for Research Resources, NIH [RR-15301]
- DOE [DE-AC02-06CH11357]
- Washington State Life Sciences Discovery Fund
- Center for the Intracellular Delivery of Biologics
- Howard Hughes Medical Institute (HHMI)
- International Aids Vaccine Initiative
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We describe a general computational method for designing proteins that self-assemble to a desired symmetric architecture. Protein building blocks are docked together symmetrically to identify complementary packing arrangements, and low-energy protein-protein interfaces are then designed between the building blocks in order to drive self-assembly. We used trimeric protein building blocks to design a 24-subunit, 13-nm diameter complex with octahedral symmetry and a 12-subunit, 11-nm diameter complex with tetrahedral symmetry. The designed proteins assembled to the desired oligomeric states in solution, and the crystal structures of the complexes revealed that the resulting materials closely match the design models. The method can be used to design a wide variety of self-assembling protein nanomaterials.
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