Journal
SCIENCE
Volume 339, Issue 6115, Pages 82-85Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1228985
Keywords
-
Categories
Funding
- Excellence Cluster Center for integrated Protein Science Munich [Exc114/1]
- European Molecular Biology Organization
- Deutsche Forschungsgemeinschaft [JU270/5-3, WI3285/1-1]
- AXA Postdoctoral fellowship
Ask authors/readers for more resources
Translation elongation factor P (EF-P) is critical for virulence in bacteria. EF-P is present in all bacteria and orthologous to archaeal and eukaryotic initiation factor 5A, yet the biological function has so far remained enigmatic. Here, we demonstrate that EF-P is an elongation factor that enhances translation of polyproline-containing proteins: In the absence of EF-P, ribosomes stall at polyproline stretches, whereas the presence of EF-P alleviates the translational stalling. Moreover, we demonstrate the physiological relevance of EF-P to fine-tune the expression of the polyproline-containing pH receptor CadC to levels necessary for an appropriate stress response. Bacterial, archaeal, and eukaryotic cells have hundreds to thousands of polyproline-containing proteins of diverse function, suggesting that EF-P and a/eIF-5A are critical for copy-number adjustment of multiple pathways across all kingdoms of life.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available