Journal
SCIENCE
Volume 338, Issue 6105, Pages 379-383Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1217726
Keywords
-
Categories
Funding
- Swiss National Science Foundation [SNF 31-113565, SNF 31-128656/1]
- European Research Council [ERC-2009-AdG 20090506, ERC-2010-StG-260667]
- State of Geneva
- Louis Jeantet Foundation of Medicine
- Smoking Research Foundation of Japan
- Swiss National Science Foundation (SNF) [31-130714]
- Ecole Polytechnique Federale de Lausanne (EPFL)
- Grants-in-Aid for Scientific Research [24592446] Funding Source: KAKEN
Ask authors/readers for more resources
In mammalian tissues, circadian gene expression can be driven by local oscillators or systemic signals controlled by the master pacemaker in the suprachiasmatic nucleus. We show that simulated body temperature cycles, but not peripheral oscillators, controlled the rhythmic expression of cold-inducible RNA-binding protein (CIRP) in cultured fibroblasts. In turn, loss-of-function experiments indicated that CIRP was required for high-amplitude circadian gene expression. The transcriptome-wide identification of CIRP-bound RNAs by a biotin-streptavidin-based cross-linking and immunoprecipitation (CLIP) procedure revealed several transcripts encoding circadian oscillator proteins, including CLOCK. Moreover, CLOCK accumulation was strongly reduced in CIRP-depleted fibroblasts. Because ectopic expression of CLOCK improved circadian gene expression in these cells, we surmise that CIRP confers robustness to circadian oscillators through regulation of CLOCK expression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available