Journal
SCIENCE
Volume 338, Issue 6113, Pages 1469-1472Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1228110
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Funding
- Austrian Science Fund FWF [F4302-B09, W1207-B09]
- Genome Research in Austria [GEN-AU 820980]
- Austrian Science Fund (FWF) [W1207] Funding Source: Austrian Science Fund (FWF)
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Mammalian imprinted genes often cluster with long noncoding (lnc) RNAs. Three lncRNAs that induce parental-specific silencing show hallmarks indicating that their transcription is more important than their product. To test whether Airn transcription or product silences the Igf2r gene, we shortened the endogenous lncRNA to different lengths. The results excluded a role for spliced and unspliced Airn lncRNA products and for Airn nuclear size and location in silencing Igf2r. Instead, silencing only required Airn transcriptional overlap of the Igf2r promoter, which interferes with RNA polymerase II recruitment in the absence of repressive chromatin. Such a repressor function for lncRNA transcriptional overlap reveals a gene silencing mechanism that may be widespread in the mammalian genome, given the abundance of lncRNA transcripts.
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