Journal
SCIENCE
Volume 335, Issue 6076, Pages 1621-1623Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1214402
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Funding
- UK Biotechnology and Biological Sciences Research Council (BBSRC) [BB/D010799/1]
- EU [LSHG-CT-2006-037900]
- BBSRC [BB/G01406X/1, BBS/E/J/000CA447, BB/D010799/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G01406X/1, BB/D010799/1, BBS/E/J/000CA447] Funding Source: researchfish
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Transcription termination is emerging as an important component of gene regulation necessary to partition the genome and minimize transcriptional interference. We have discovered a role for the Arabidopsis RNA silencing enzyme DICER-LIKE 4 (DCL4) in transcription termination of an endogenous Arabidopsis gene, FCA. DCL4 directly associates with FCA chromatin in the 3' region and promotes cleavage of the nascent transcript in a domain downstream of the canonical polyA site. In a dcl4 mutant, the resulting transcriptional read-through triggers an RNA interference-mediated gene silencing of a transgene containing the same 3' region. We conclude that DCL4 promotes transcription termination of the Arabidopsis FCA gene, reducing the amount of aberrant RNA produced from the locus.
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