Journal
SCIENCE
Volume 333, Issue 6047, Pages 1307-1311Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1205527
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Funding
- Bauer Fellows program
- National Institute of General Medical Sciences [GM068763]
- U.S. Army [W81XWH-09-1-0240 BC085163]
- National Cancer Institute [1R01CA155320-01]
- ETH Zurich
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Engineered biological systems that integrate multi-input sensing, sophisticated information processing, and precisely regulated actuation in living cells could be useful in a variety of applications. For example, anticancer therapies could be engineered to detect and respond to complex cellular conditions in individual cells with high specificity. Here, we show a scalable transcriptional/posttranscriptional synthetic regulatory circuit-a cell-type classifier-that senses expression levels of a customizable set of endogenous microRNAs and triggers a cellular response only if the expression levels match a predetermined profile of interest. We demonstrate that a HeLa cancer cell classifier selectively identifies HeLa cells and triggers apoptosis without affecting non-HeLa cell types. This approach also provides a general platform for programmed responses to other complex cell states.
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