Journal
SCIENCE
Volume 332, Issue 6030, Pages 732-735Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1198851
Keywords
-
Categories
Funding
- ICREA Academia (Generalitat de Catalunya)
- Fundacion Marcelino Botin (FMB)
- Ministerio de Ciencia y Innovacion [BFU2008-00530, BIO2009-07762]
- Swiss National Science Foundation
- ETHZ
- [155111]
Ask authors/readers for more resources
Mitogen-activated protein kinase (MAPK) cascades are conserved signaling modules that control many cellular processes by integrating intra- and extracellular cues. The p38/Hog1 MAPK is transiently activated in response to osmotic stress, leading to rapid translocation into the nucleus and induction of a specific transcriptional program. When investigating the dynamic interplay between Hog1 activation and Hog1-driven gene expression, we found that Hog1 activation increases linearly with stimulus, whereas the transcriptional output is bimodal. Modeling predictions, corroborated by single-cell experiments, established that a slow stochastic transition from a repressed to an activated transcriptional state in conjunction with transient Hog1 activation generates this behavior. Together, these findings provide a molecular mechanism by which a cell can impose a transcriptional threshold in response to a linear signaling behavior.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available