Journal
SCIENCE
Volume 335, Issue 6065, Pages 221-225Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1215804
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Funding
- NIH [GM094522, GM08295]
- NSF [MCB-1055017]
- Burroughs Wellcome Foundation
- Direct For Biological Sciences [1055017] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience [1055017] Funding Source: National Science Foundation
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Cytoplasmic dynein is a homodimeric AAA+ motor that transports a multitude of cargos toward the microtubule minus end. How the two catalytic head domains interact and move relative to each other during processive movement is unclear. Here, we tracked the relative positions of both heads with nanometer precision and directly observed the heads moving independently along the microtubule. The heads remained widely separated, and their stepping behavior varied as a function of interhead separation. One active head was sufficient for processive movement, and an active head could drag an inactive partner head forward. Thus, dynein moves processively without interhead coordination, a mechanism fundamentally distinct from the hand-over-hand stepping of kinesin and myosin.
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