Journal
SCIENCE
Volume 334, Issue 6057, Pages 817-821Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1213671
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Funding
- NIH [5T32-GM068411, R01-GM34277, R01-CA133404, R01-GM52347]
- Cancer Center from the National Cancer Institute [P30-CA14051]
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The CCA-adding enzyme [ATP(CTP): tRNA nucleotidyltransferase] adds CCA to the 3' ends of transfer RNAs (tRNAs), a critical step in tRNA biogenesis that generates the amino acid attachment site. We found that the CCA-adding enzyme plays a key role in tRNA quality control by selectively marking structurally unstable tRNAs and tRNA-like small RNAs for degradation. Instead of adding CCA to the 3' ends of these transcripts, CCA-adding enzymes from all three kingdoms of life add CCACCA. In addition, hypomodified mature tRNAs are subjected to CCACCA addition as part of a rapid tRNA decay pathway in vivo. We conjecture that CCACCA addition is a universal mechanism for controlling tRNA levels and preventing errors in translation.
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