Journal
SCIENCE
Volume 331, Issue 6022, Pages 1328-1332Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1201538
Keywords
-
Categories
Funding
- European Research Council [202337]
- Royal Society
- Spanish government [BFU2008-00408/BMC]
- Cancer Research UK
- MRC UK
- MRC [MC_U120074328] Funding Source: UKRI
- European Research Council (ERC) [202337] Funding Source: European Research Council (ERC)
- Medical Research Council [G0801130B, MC_U120074328] Funding Source: researchfish
Ask authors/readers for more resources
DNA topoisomerase II completely removes DNA intertwining, or catenation, between sister chromatids before they are segregated during cell division. How this occurs throughout the genome is poorly understood. We demonstrate that in yeast, centromeric plasmids undergo a dramatic change in their topology as the cells pass through mitosis. This change is characterized by positive supercoiling of the DNA and requires mitotic spindles and the condensin factor Smc2. When mitotic positive supercoiling occurs on decatenated DNA, it is rapidly relaxed by topoisomerase II. However, when positive supercoiling takes place in catenated plasmid, topoisomerase II activity is directed toward decatenation of the molecules before relaxation. Thus, a topological change on DNA drives topoisomerase II to decatenate molecules during mitosis, potentially driving the full decatenation of the genome.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available