Journal
SCIENCE
Volume 333, Issue 6041, Pages 470-474Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1206938
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Funding
- Enerbio-Tuck Foundation
- Institut Francais du Petrole Energies Nouvelles
- Agence Nationale de la Recherche France
- Institut National de la Sante et de la Recherche Medicale-Institut National de Recherche en Informatique et en Automatique projet d'envergure
- Axa Foundation Chair on Longevity
- Natural Sciences and Engineering Research Council of Canada
- Wyss Institute for Biologically Inspired Engineering
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The rules of nucleic acid base-pairing have been used to construct nanoscale architectures and organize biomolecules, but little has been done to apply this technology in vivo. We designed and assembled multidimensional RNA structures and used them as scaffolds for the spatial organization of bacterial metabolism. Engineered RNA modules were assembled into discrete, one-dimensional, and two-dimensional scaffolds with distinct protein-docking sites and used to control the spatial organization of a hydrogen-producing pathway. We increased hydrogen output as a function of scaffold architecture. Rationally designed RNA assemblies can thus be used to construct functional architectures in vivo.
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