Journal
SCIENCE
Volume 327, Issue 5966, Pages 685-689Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1182105
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Funding
- European Research
- European Molecular Biology Organisation
- Balliol College
- Oxford University
- Royal Society
- Engineering and Physical Sciences Research Council
- Biotechnology and Biological Sciences Research Council [BB/H01991X/1, BB/E00458X/1] Funding Source: researchfish
- BBSRC [BB/E00458X/1, BB/H01991X/1] Funding Source: UKRI
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The bacterial flagellar switch that controls the direction of flagellar rotation during chemotaxis has a highly cooperative response. This has previously been understood in terms of the classic two-state, concerted model of allosteric regulation. Here, we used high-resolution optical microscopy to observe switching of single motors and uncover the stochastic multistate nature of the switch. Our observations are in detailed quantitative agreement with a recent general model of allosteric cooperativity that exhibits conformational spread-the stochastic growth and shrinkage of domains of adjacent subunits sharing a particular conformational state. We expect that conformational spread will be important in explaining cooperativity in other large signaling complexes.
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