Journal
SCIENCE
Volume 324, Issue 5933, Pages 1451-1454Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1172417
Keywords
-
Categories
Funding
- NIH [GM69415]
- fellow of the Jane Coffin Childs Memorial Fund for Medical Research
Ask authors/readers for more resources
Parent-of-origin-specific (imprinted) gene expression is regulated in Arabidopsis thaliana endosperm by cytosine demethylation of the maternal genome mediated by the DNA glycosylase DEMETER, but the extent of the methylation changes is not known. Here, we show that virtually the entire endosperm genome is demethylated, coupled with extensive local non-CG hypermethylation of small interfering RNA-targeted sequences. Mutation of DEMETER partially restores endosperm CG methylation to levels found in other tissues, indicating that CG demethylation is specific to maternal sequences. Endosperm demethylation is accompanied by CHH hypermethylation of embryo transposable elements. Our findings demonstrate extensive reconfiguration of the endosperm methylation landscape that likely reinforces transposon silencing in the embryo.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available